Generator

Part:BBa_I760001:Design

Designed by: Alexej Skvortsov   Group: iGEM07_Saint_Petersburg   (2007-06-27)


ech protein generator for vanillin biosynthesis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 892
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 892
    Illegal NotI site found at 836
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 892
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 892
    Illegal NgoMIV site found at 265
    Illegal NgoMIV site found at 283
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Needs to be used in conjuction with fcs in order to generate vanillin


Source

uses BBa_I760000, promotor, RBS and terminator. Promotor is EPTG inducible. Contains high efficiency RBS and terminator.

References